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KMID : 1007520180270030883
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Food Science and Biotechnology
2018 Volume.27 No. 3 p.883 ~ p.889
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Prevalence of Clostridium difficile isolated from various raw meats in Korea
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Lee Joo-Young
Lee Da-Yeon
Cho Yong-Sun
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Abstract
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Clostridium difficile is an anaerobic, toxin-producing pathogen that causes human infection possibly through the consumption of meat. Clostridium difficile was isolated from 45 of 415 (10.8%) various raw meat samples collected in nationwide markets in Korea between 2013 and 2014. Among the 45 isolated strains, the highest prevalence rate was found in September (28.6%) and detected in chicken (16.4%), pork (8.3%) and beef (6.8%). According to an antibiotic resistance test, resistance was found only for clindamycin (2.2%). The genetic similarity of ribotypes O78 and O27 and strains isolated from raw meats was determined using DiversiLab. Among the isolates studied, four different rep-PCR types were identified, genetically distinct from ribotypes O78 and O27. An ELISA reaction confirmed that the two strains have toxin A and toxin B and showed 89% genetic similarity. This study suggests that food animals could be potential routes of foodborne transmission in C. difficile-associated human infection.
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KEYWORD
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Clostridium difficile, Toxin, Ribotypes, Antimicrobial resistance, Raw meat
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